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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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| Cell line name | PA317 |
| Accession number | ICLC HTL06006 |
| Brief description | Species: mouse, NIH Swiss; Tissue: embryo; Transfected with: (pPAM3) and HSV thymidine kinase; Original line: TK- NIH/3T3 cells |
| Description | Species: mouse, NIH Swiss; Tissue: embryo; cotransfection with packaging construct DNA (pPAM3) carried in pBR322 and the herpes simplex virus thymidine kinase (TK) gene carried in pBR322.; Original line: TK- NIH/3T3 cells |
| Depositor | Dr M. Dominici, Oncology Dept, University of Modena e Reggio Emilia, Italy |
| Reference paper | J Biol Chem 1996;271: 13968-13975 - DOI: 10.1074/jbc.271.24.13968 - PMID: 8662928 |
| Morphology and growth | continuous culture, grown as monolayer, morphology fibroblast |
| Culture conditions | DMEM (4.5g/l glucose) + 10% FBS + 2mM L-Glutamine; split confluent cultures 1:5-1:10 using trypsin/EDTA, 37C, 5% CO2 |
| Properties | Packaging line for retroviruses |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | Handle under Biosafety Level 2 containment |
| Passage number | 3 |
| Freezing medium | Culture medium + 5% DMSO |
| Comments | amphotropic packaging cell line |