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Cell line namePA317
Accession numberICLC HTL06006
Brief descriptionSpecies: mouse, NIH Swiss; Tissue: embryo; Transfected with: (pPAM3) and HSV thymidine kinase; Original line: TK- NIH/3T3 cells
DescriptionSpecies: mouse, NIH Swiss; Tissue: embryo; cotransfection with packaging construct DNA (pPAM3) carried in pBR322 and the herpes simplex virus thymidine kinase (TK) gene carried in pBR322.; Original line: TK- NIH/3T3 cells
DepositorDr M. Dominici, Oncology Dept, University of Modena e Reggio Emilia, Italy
Reference paper J Biol Chem 1996;271: 13968-13975 - PMID: 8662928
Morphology and growthcontinuous culture, grown as monolayer, morphology fibroblast
Culture conditionsDMEM (4.5g/l glucose) + 10% FBS + 2mM L-Glutamine; split confluent cultures 1:5-1:10 using trypsin/EDTA, 37C, 5% CO2
PropertiesPackaging line for retroviruses
DistributionCell line available for distribution. For non-commercial investigative use only
HazardHandle under Biosafety Level 2 containment
Passage number3
Freezing mediumCulture medium + 5% DMSO
Commentsamphotropic packaging cell line

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