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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | GPE 86 |
| Accession number | ICLC HTL06007 |
| Brief description | Species: mouse, NIH Swiss; Tissue: kidney, embryonic |
| Description | Species: mouse, NIH Swiss; Tissue: kidney, embryonic |
| Depositor | Dr M. Dominici, Oncology Dept, University of Modena e Reggio Emilia, Italy |
| Reference paper | Blood 1997 Sep 1;90(5):1777-86 - PMID: 9292510 |
| Morphology and growth | continuous culture, grown as monolayer, morphology fibroblast |
| Culture conditions | DMEM (4.5g/l glucose) + 10% FBS + 2mM L-Glutamine; split cultures 1:5-1:10 using trypsin/EDTA, 37C, 5% CO2 |
| Properties | Cell line can be used to produce retroviral vector |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | Handle under Biosafety Level 2 containment |
| Passage number | 6 |
| Freezing medium | Culture medium + 5% DMSO |
| Validation assays | Multiplex PCR: tested against human, rat, mouse primers. Confirmed as mouse with cytochrome c oxidase subunit I (cox I) primers |
| Comments | Ecotropic packaging cell line |