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Cell line nameGPE 86
Accession numberICLC HTL06007
Brief descriptionSpecies: mouse, NIH Swiss; Tissue: kidney, embryonic
DescriptionSpecies: mouse, NIH Swiss; Tissue: kidney, embryonic
DepositorDr M. Dominici, Oncology Dept, University of Modena e Reggio Emilia, Italy
Reference paperBlood 1997 Sep 1;90(5):1777-86 - PMID: 9292510
Morphology and growthcontinuous culture, grown as monolayer, morphology fibroblast
Culture conditionsDMEM (4.5g/l glucose) + 10% FBS + 2mM L-Glutamine; split cultures 1:5-1:10 using trypsin/EDTA, 37C, 5% CO2
PropertiesCell line can be used to produce retroviral vector
DistributionCell line available for distribution. For non-commercial investigative use only
HazardHandle under Biosafety Level 2 containment
Passage number6
Freezing mediumCulture medium + 5% DMSO
Validation assaysMultiplex PCR: tested against human, rat, mouse primers. Confirmed as mouse with cytochrome c oxidase subunit I (cox I) primers
CommentsEcotropic packaging cell line

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