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Cell line nameMeCo 05
Accession numberICLC HTL07001
Brief descriptionSpecies: human; Tissue: skin; Tumor: melanoma
DescriptionSpecies: human male 65 year old; Tissue: skin; Tumor: melanoma; Derived from: a lymph node metastasis
DepositorDr G. Ferlazzo, Human Pathology Dept., Pad-H, University of Messina, Italy
Reference paperUnknown
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial
Culture conditionsRPMI 1640 + 10% FBS; split confluent cultures 1:3 - 1:6 using trypsin/EDTA; 37C, 5% CO2
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Passage number15
Freezing mediumCulture medium + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Multiplex PCR: tested against human, rat, mouse primers. Confirmed as human with cytochrome c oxidase subunit I (cox I) primers

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