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Cell line nameMeMo 05
Accession numberICLC HTL07002
Brief descriptionSpecies: human; Tissue: lymph node, metastasis; Tumor: melanoma
DescriptionSpecies: human male 66 years old; Tissue: lymph node, metastasis; Tumor: melanoma
DepositorDr G. Ferlazzo, Human Pathology Dept., Pad-H, University of Messina, Italy
Reference paperUnknown
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial
Culture conditionsRPMI 1640 + 10% FBS + 2mM L-Glutamine; 37C, 5% CO2, split confluent cultures 1:3 - 1:4 using trypsin/EDTA
DistributionCell line available for distribution. For non-commercial investigative use only
HazardHandle under Biosafety Level 2 containment
Passage number19
Freezing mediumCulture medium 45% + 50% FCS + 5% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Multiplex PCR: tested against human, rat, mouse primers. Confirmed as human with cytochrome c oxidase subunit I (cox I) primers
CommentsOriginator of cell line: Dr P. Carrega

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