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Cell line nameA2058
Accession numberICLC HTL08002
Brief descriptionSpecies: human, Caucasian; Tissue: skin; Tumor: melanoma, metastatic
DescriptionSpecies: human, Caucasian male 43 years old; Tissue: skin; Tumor: melanoma, metastatic; Derived from: lymph node metastasis
DepositorDr F. Tosetti, Cellular Biology Dept, IST at ABC, Genoa, Italy
Reference paperUnknown
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial
Culture conditionsRPMI 1640 + 10% FBS + 2mM-Glutamine + 4,5 g/L glucose; split confluent cultures1:3-1:6; 37C, 5% CO2
DistributionCell line available for distribution. For non-commercial investigative use only
Freezing mediumCulture medium 45% + 50% FCS + 5% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Multiplex PCR: tested against human, rat, mouse primers. Confirmed as human with cytochrome c oxidase subunit I (cox I) primers
Further bibliographyProc Natl Acad Sci U S A. 1977;74(2):565-9 - DOI: 10.1073/pnas.74.2.565 - PMID: 265522
Proc Natl Acad Sci U S A. 1979; 76(3):1288-92 - DOI: 10.1073/pnas.76.3.1288 - PMID: 375235
Proc Natl Acad Sci U S A. 1980;77(9):5258-62 - DOI: 10.1073/pnas.77.9.5258 - PMID: 6254071

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