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Cell line nameU87/DK
Accession numberICLC HTL08003
Brief descriptionSpecies: human; Tissue: brain; Tumor: glioblastoma; Transfected with: binase (ATP binding site 721), mutated de 2-7 EGFR
DescriptionSpecies: human; Tissue: brain; Tumor: glioblastoma; Transfected with binase (ATP binding site 721), mutated de 2-7 EGFR
DepositorDr T. Florio, DOBIG, Pharmacology Dept, University of Genoa, Italy,
Reference paperUnknown
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial like
Culture conditionsDMEM + 10% FBS + 2mM L-Glutamine; split 1:3-1:6 using trypsin/EDTA; 37C, 5% CO2
DistributionCell line available for distribution. For non-commercial investigative use only
HazardHandle under Biosafety Level 2 containment
Freezing mediumCulture medium 45% + 50% FCS + 5% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Multiplex PCR: tested against human, rat, mouse primers. Confirmed as human with cytochrome c oxidase subunit I (cox I) primers

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