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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | U87/DK |
| Accession number | ICLC HTL08003 |
| Brief description | Species: human; Tissue: brain; Tumor: glioblastoma; Transfected with: binase (ATP binding site 721), mutated de 2-7 EGFR |
| Description | Species: human; Tissue: brain; Tumor: glioblastoma; Transfected with binase (ATP binding site 721), mutated de 2-7 EGFR |
| Depositor | Dr T. Florio, DOBIG, Pharmacology Dept, University of Genoa, Italy, |
| Reference paper | Unknown |
| Morphology and growth | continuous culture, grown as monolayer, morphology epithelial like |
| Culture conditions | DMEM + 10% FBS + 2mM L-Glutamine; split 1:3-1:6 using trypsin/EDTA; 37C, 5% CO2 |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | Handle under Biosafety Level 2 containment |
| Freezing medium | Culture medium 45% + 50% FCS + 5% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. Multiplex PCR: tested against human, rat, mouse primers. Confirmed as human with cytochrome c oxidase subunit I (cox I) primers |