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Cell line nameU87/WT
Accession numberICLC HTL08004
Brief descriptionSpecies: human; Tissue: brain; Tumor: glioblastoma; Transfected with: Transfected with wt EGFR
DescriptionSpecies: human; Tissue: brain; Tumor: glioblastoma; Transfected with: Transfected with wt EGFR
DepositorDr T. Florio, DOBIG, Pharmacology Dept, University of Genoa, Italy,
Reference paperProc Natl Acad Sci U S A. 1994 Aug 2;91(16):7727-31 - PMID: 8052651
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial-like
Culture conditionsDMEM + 10% FBS + 2mM L-Glutamine; Split confluent cultures 1:3-1:6 using trypsin/EDTA; 37C, 5% CO2
DistributionCell line available for distribution. For non-commercial investigative use only
HazardHandle as Biosafety Level 2 containment
Freezing mediumCulture medium 45% + 50% FCS + 5% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Multiplex PCR: tested again human, rat, mouse primers. Confirmed as human with cytochrome c oxidase subunit I (cox I) primers

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