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Cell line nameSK-HEP-1
Accession numberICLC HTL10001
Brief descriptionSpecies: human, Caucasian; Tissue: liver; Tumor: adenocarcinoma
DescriptionSpecies: human, Caucasian male; Tissue: liver; Tumor: adenocarcinoma
DepositorA M Bassi e S Penco, DIMES, General Pathology, Cell Cultures Lab, University of Genoa, Italy
Reference paperUnknown
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial-like
Culture conditionsMEM (EBSS) + 10% FBS + 1% non-essential amino acids; split confluent cultures 1:5 - 1:10 using trypsin/EDTA, 37C, 5% CO2
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Freezing mediumCulture medium 45% + 50% FCS + 5% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Multiplex PCR: tested against human, rat, mouse primers. Confirmed as human with cytochrome c oxidase subunit I (cox I) primers

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