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Cell line nameMDA-MB-436
Accession numberICLC HTL11005
Brief descriptionSpecies: human, Caucasian; Tissue: mammary gland; Tumor: adenocarcinoma, breast
DescriptionSpecies: human, Caucasian female 43 years old; Tissue: mammary gland; Tumor: adenocarcinoma, breast; Derived from: pleural effusion
DepositorDr. A. Garuti, Cell Therapy, DIMI, Genoa, Italy
Reference paperCancer Res 1979;39: 919-922 - PMID: 427779
Morphology and growthcontinuous culture, grown as monolayer, morphology pleomorphic with multinucleated component cells
Culture conditionsRPMI 1640 + 10% FBS + 2mM L-Glutamine; 37C, 5% CO2, split cultures 1:2-1:5 using trypsin/EDTA
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Passage number8
Freezing mediumCulture medium 45% + 50% FCS + 5% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.

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