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Cell line nameBPH-1
Accession numberICLC HTL11006
Brief descriptionSpecies: human; Tissue: prostate
DescriptionSpecies: human male 68 years old; Tissue: prostate; Derived from: benign prostate epithelial cells hyperplasia
DepositorDr. G. Angelini, IRCCS AOU San Martino - IST, Genoa, Italy
Reference paperUnknown
Morphology and growthcontinuous culture, grown as monolayer, morphology epitheloid cells
Culture conditionsRPMI 1640 + 20% FBS; 37C, 5% CO2, split cultures 1:3-1:6 every 3-7 days using trypsin/EDTA
Propertiescells were described to express cytokeratins 8, 18, and 19 (but not 14) and to metabolize prostatic androgens
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Freezing medium95% FCS + 5% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Further bibliography In Vitro Cell Dev Biol Anim 1995;31(1):14-24. - PMID: 7535634
Commentscells were immortalized with SV-40 large T-antigen

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