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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | BPH-1 |
| Accession number | ICLC HTL11006 |
| Brief description | Species: human; Tissue: prostate |
| Description | Species: human male 68 years old; Tissue: prostate; Derived from: benign prostate epithelial cells hyperplasia |
| Depositor | Dr. G. Angelini, IRCCS AOU San Martino - IST, Genoa, Italy |
| Reference paper | Unknown |
| Morphology and growth | continuous culture, grown as monolayer, morphology epitheloid cells |
| Culture conditions | RPMI 1640 + 20% FBS; 37C, 5% CO2, split cultures 1:3-1:6 every 3-7 days using trypsin/EDTA |
| Properties | cells were described to express cytokeratins 8, 18, and 19 (but not 14) and to metabolize prostatic androgens |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Freezing medium | 95% FCS + 5% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |
| Further bibliography | In Vitro Cell Dev Biol Anim 1995;31(1):14-24. - DOI: 10.1007/BF02631333
- PMID: 7535634
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| Comments | cells were immortalized with SV-40 large T-antigen |