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Cell line nameCALOGERO
Accession numberICLC HTL14001
Brief descriptionSpecies: human; Tissue: lymphocyte, B; Transformed by: Epstein-Barr
DescriptionSpecies: human male; Tissue: lymphocyte, B; Transformed by: Epstein-Barr
DepositorDr. M. P. Pistillo, IRCCS AOU San Martino - IST, Genoa, Italy
Reference paperTissue Antigens. 1996 Jun;47(6):538-46. - PMID: 8813743
Morphology and growthcontinuous culture, grown in suspension, morphology lymphoblast like
Culture conditionsRPMI 1640 + 10% FBS + 2mM L-Glutamine; Split 1:2 -1:3 twice weekly. 37C, 5% CO2
DistributionCell line available for distribution. For non-commercial investigative use only
HazardHandle under Biosafety Level 2 containment
Passage number> 20
Freezing medium95% FCS + 5% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
HLA typing: A2; Cw2; B61; Bw6; DR16; DQ5. A*0201; Cw*2022; B*4002; DQA1*0102; DPB1*0401
CommentsOriginator of cell line: Dr. A. Cambon Thomsen. Cell line from the 10th International Histocompatibility Workshop Cell Panel, (10IHW). 21-Hydroxylase Deficiency

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