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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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| Cell line name | CALOGERO |
| Accession number | ICLC HTL14001 |
| Brief description | Species: human; Tissue: lymphocyte, B; Transformed by: Epstein-Barr |
| Description | Species: human male; Tissue: lymphocyte, B; Transformed by: Epstein-Barr |
| Depositor | Dr. M. P. Pistillo, IRCCS AOU San Martino - IST, Genoa, Italy |
| Reference paper | Tissue Antigens. 1996 Jun;47(6):538-46. - DOI: 10.1111/j.1399-0039.1996.tb02596.x - PMID: 8813743 |
| Morphology and growth | continuous culture, grown in suspension, morphology lymphoblast like |
| Culture conditions | RPMI 1640 + 10% FBS + 2mM L-Glutamine; Split 1:2 -1:3 twice weekly. 37C, 5% CO2 |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | Handle under Biosafety Level 2 containment |
| Passage number | > 20 |
| Freezing medium | 95% FCS + 5% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. HLA typing: A2; Cw2; B61; Bw6; DR16; DQ5. A*0201; Cw*2022; B*4002; DQA1*0102; DPB1*0401 |
| Comments | Originator of cell line: Dr. A. Cambon Thomsen. Cell line from the 10th International Histocompatibility Workshop Cell Panel, (10IHW). 21-Hydroxylase Deficiency |