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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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| Cell line name | BOB |
| Accession number | ICLC HTL14003 |
| Brief description | Species: human; Tissue: lymphocyte, B; Transformed by: Epstein-Barr |
| Description | Species: human female; Tissue: lymphocyte, B; Transformed by: Epstein-Barr |
| Depositor | Dr. M. P. Pistillo, IRCCS AOU San Martino - IST, Genoa, Italy |
| Reference paper | Tissue Antigens. 1996 Jun;47(6):538-46 - DOI: 10.1111/j.1399-0039.1996.tb02596.x - PMID: 8813743 |
| Morphology and growth | continuous culture, grown in suspension, morphology lymphoblast like |
| Culture conditions | RPMI 1640 + 10% FBS + 2mM L-Glutamine; Split 1:2. 37C, 5% CO2 |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | Handle under Biosafety Level 2 containment |
| Passage number | >20 |
| Freezing medium | 95% FCS + 5% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. HLA typing: A24; B51; Bw4; DR11; DRw52; DQ7. A*2402; Cw*1502; B*5101; DRA*0101; DRB1*1104; DRB3*0202; DQA1*0501; DQB1*0301; DPA1*01; DPB1*0402 |
| Comments | Originator of cell line: Dr. C. Muller. Cell line from the 10th International Histocompatibility Workshop Cell Panel. (10IHW) |