 |
Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
||
|
Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
||
| Cell line name | PF97387 |
| Accession number | ICLC HTL14004 |
| Brief description | Species: human; Tissue: lymphocyte, B; Transformed by: Epstein-Barr |
| Description | Species: human male; Tissue: lymphocyte, B; Transformed by: Epstein-Barr |
| Depositor | Dr. M. P. Pistillo, IRCCS AOU San Martino - IST, Genoa, Italy |
| Reference paper | Tissue Antigens. 1996 Jun;47(6):538-46 - PMID: 881374 |
| Morphology and growth | continuous culture, grown in suspension, morphology lymphoblast like |
| Culture conditions | RPMI 1640 + 10% FBS + 2mM L-Glutamine; Split 1:2-1:3 twice weekly. 37C, 5% CO2 |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | Handle under Biosafety Level 2 containment |
| Passage number | > 20 |
| Freezing medium | 95% FCS + 5% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. HLA typing: A29; B44; Bw4; DR4; DRw53; DQ7. A*2902; Cw*1603; B*44031; DRB1*04; DQA1*03; DQB1*0301; DPA1*01; DPB1*0401; DPB1*0402; MICA*004 |
| Comments | Originator of cell line: Prof. R. Fauchet. Cell line from the 10th International Histocompatibility Workshop Cell Panel (10IHW). |