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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | K-562 |
| Accession number | ICLC HTL94001 |
| Brief description | Species: human, Caucasian; Tissue: pleural effusion; Tumor: leukemia, myelogenous |
| Description | Species: human, Caucasian female 53 years old; Tissue: pleural effusion; Tumor: leukemia, myelogenous |
| Depositor | Dr V. Dessi, San Martino Hospital, Genoa, Italy |
| Reference paper | Blood 1975;45:321-334 - PMID: 163658 |
| Morphology and growth | continuous culture, grown in suspension, morphology round and large cells |
| Culture conditions | RPMI 1640 + 10% FBS + 2mM L-Glutamine; Maintain cultures between 1 and 5x105 cells/ml; 37C, 5% CO2 |
| Properties | haemoglobin synthesis; antitumour testing; tumorigenicity; differentiation; pharmacodynamics; cloning; cytotoxicity; cellular effects of hyperthermia; natural killer assay |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as human with AST, NP, Pep B |
| Validated by HLA-DP beta: confirmed as human by PCR | |
| Tumorigenicity | tumorigenic in nude mice |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |
| Further bibliography | Blood 1980;56:344-350 - PMID: 6996765
Clin Haematol 1984;13:461-486 - PMID: 6088146 J Immunol 1982;129:2504-2510 - PMID: 6982930 |