ICLC logo Cell bank
IRCCS AOU San Martino IST
Interlab Cell Line Collection (ICLC)
On-Line Catalogue

Home - Search by name - Free text search - Search by properties

Cell line nameU-937
Accession numberICLC HTL94002
Brief descriptionSpecies: human, Caucasian; Tissue: pleural effusion; Tumor: lymphoma, histiocytic
DescriptionSpecies: human, Caucasian male 37 years old; Tissue: pleural effusion; Tumor: lymphoma, histiocytic
DepositorObtained from ATCC. Established by C. Sundrom and K. Nilsson in 1974.
Reference paperInt J Cancer 1976;17:565-577 - PMID: 178611
Morphology and growthcontinuous culture, grown in suspension, morphology histomonocytoid
Culture conditionsRPMI 1640 + 10% FBS + 2mM L-Glutamine; Split 1:5-1:20, maintain cultures between 2 and 9x105 cells/ml. 37C, 5% CO2.
PropertiesIL-1 production; specialized product secretion; tumor markers; Fc receptors; antitumour testing; virology; tumorigenicity; expression of markers and properties of monocytes, expression of mRNA for c-myc.; Susceptible to: HIV-1, HIV-2
DistributionCell line available for distribution. The cell line must not be exploited commercially
Hazard
Species validationValidated by isoenzymes: confirmed as human with AST, NP, PepB
Validated by HLA-DP beta: confirmed as human by PCR
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Amelogenin: X; vWA: 14,15,16,17; FGA: 22,25; TH01: 6,9.3; D18S51: 13,14; D21S11: 27,29; D8S1179: 12,13
Further bibliographyJ Exp Med 1976;143:1528-1533 - PMID: 1083890
J Immunol 1980;125:463-465 - PMID: 7381208
Nature 1979;279:328-331 - PMID: 450085

For any info contact Interlab Cell Line Collection
HTML by Paolo Romano