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Cell line nameCalu-1
Accession numberICLC HTL95002
Brief descriptionSpecies: human, Caucasian; Tissue: lung; Tumor: carcinoma, epidermoid, grade III
DescriptionSpecies: human, Caucasian male 47 years old; Tissue: lung; Tumor: carcinoma, epidermoid, grade III; Derived from: metastasis to pleura
DepositorObtained from ECACC.
Reference paperHuman tumor cells in vitro. Fogh J. ed., Plenum Press, New York, 1975; pp. 115-159
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial-like
Culture conditionsMEM (EBSS) + 10% FBS + 2mM L-Glutamine + 1mM Na pyruvate + 1% non-essential amino acids; Split confluent cultures 1:2-1:4 using trypsin/EDTA; seed at 3-5 x 104 cells/cm2. 37C, 5% CO2
PropertiesHLA: A10, A11; B15, Bw35
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Species validationValidated by isoenzymes: confirmed as human with AST, MD, NP, PepB
Passage number52
Tumorigenicitytumorigenic in nude mice
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Further bibliographyJ Natl Cancer Inst 1977;58:209-214 - PMID: 833871
Natl Cancer Inst Monogr 1978;49:5-19 - PMID: 571047

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