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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | SK-MES-1 |
| Accession number | ICLC HTL95004 |
| Brief description | Species: human; Tissue: lung; Tumor: carcinoma, squamous cell |
| Description | Species: human male 65 years old; Tissue: lung; Tumor: carcinoma, squamous cell; Derived from: pleural effusion |
| Depositor | Obtained from ECACC. |
| Reference paper | Human tumor cells in vitro. Fogh J. ed., Plenum Press, New York, 1975; pp. 115-159 - DOI: 10.1016/0006-291x(75)90293-4 |
| Morphology and growth | continuous culture, grown as monolayer, morphology epithelial-like |
| Culture conditions | MEM (HBSS) + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; Split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 3-5x104 cells/cm2. 37C, 5% CO2. |
| Properties | HLA: A3, Aw30; B7, B27 |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as human with MD, MPI |
| Karyology | hypotriploid |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |
| Further bibliography | In Vitro 1975;115 - DOI: 10.1016/0006-291x(75)90293-4
J Natl Cancer Inst 1977;58:209-214 - DOI: 10.1093/jnci/58.2.209
- PMID: 833871
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