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Cell line nameSK-MES-1
Accession numberICLC HTL95004
Brief descriptionSpecies: human; Tissue: lung; Tumor: carcinoma, squamous cell
DescriptionSpecies: human male 65 years old; Tissue: lung; Tumor: carcinoma, squamous cell; Derived from: pleural effusion
DepositorObtained from ECACC.
Reference paperHuman tumor cells in vitro. Fogh J. ed., Plenum Press, New York, 1975; pp. 115-159
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial-like
Culture conditionsMEM (HBSS) + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; Split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 3-5x104 cells/cm2. 37C, 5% CO2.
PropertiesHLA: A3, Aw30; B7, B27
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Species validationValidated by isoenzymes: confirmed as human with MD, MPI
Karyologyhypotriploid
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Further bibliographyIn Vitro 1975;115 J Natl Cancer Inst 1977;58:209-214 - PMID: 833871

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