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Cell line nameHep G2
Accession numberICLC HTL95005
Brief descriptionSpecies: human, Caucasian; Tissue: liver; Tumor: carcinoma, hepatocellular
DescriptionSpecies: human, Caucasian male 15 years old; Tissue: liver; Tumor: carcinoma, hepatocellular
DepositorProf. M. Ferro, DIMES, General Pathology, University of Genoa, Italy
Reference paperNature 1979;282:615-616 - DOI: 10.1038/282615a0 - PMID: 233137
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial
Culture conditionsMEM (EBSS) + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 2-3 x 10^4 cells/cm2; 37C, 5% CO2
Propertiesthe cells are reported to produce: alpha-fetoprotein, albumin, alpha2-macroglobulin, alpha1-antitrypsin, transferrin, alpha1-antichimotrypsin, haptoglobin, ceruloplasmin, plasminogen, C3,C4, C3 act., fibrinogen, alpha1-acid glycop., alpha2-HS glycoprotein, beta lipoprotein and retinol binding protein; xenobiotic metabolism, drug metabolism assays, toxicity screening, hepatocyte studies
DistributionCell line available for distribution. For non-commercial investigative use only
Species validationValidated by isoenzymes: confirmed as human with MD, MPI
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Amelogenin: X,Y; vWA: 17; FGA: 22,25; TH01: 9; D18S51: 13,14; D21S11: 29,31; D8S1179: 15,16
Further bibliographyScience 1980;209:497-499 - DOI: 10.1126/science.6248960 - PMID: 6248960

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