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Cell line nameHuP-T4
Accession numberICLC HTL95006
Brief descriptionSpecies: human; Tissue: pancreas; Tumor: adenocarcinoma
DescriptionSpecies: human male 60 year old; Tissue: pancreas; Tumor: adenocarcinoma; Derived from: ascitic fluid
DepositorObtained from ECACC.
Reference paperInt J Pancreatol 1993;13(1):31-41 - PMID: 8454916
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial
Culture conditionsMEM (EBSS) + 10% FBS + 2mM L-Glutamine + 1mM Na pyruvate + 1% non-essential amino acids; split confluent cultures 1:2-1:3 using trypsin/EDTA; seed at 2-4 x 104 cells/cm2; 37C, 5% CO2
PropertiesCEA and CA19-9 production
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Species validationValidated by isoenzymes: confirmed as human with MD, MPI, NP
Karyologymodal number 77
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Commentsincubate with trypsin/EDTA at 37C for at least 10-15 min. After thawing the viability of the cells may be low.

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