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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | HuP-T4 |
| Accession number | ICLC HTL95006 |
| Brief description | Species: human; Tissue: pancreas; Tumor: adenocarcinoma |
| Description | Species: human male 60 year old; Tissue: pancreas; Tumor: adenocarcinoma; Derived from: ascitic fluid |
| Depositor | Obtained from ECACC. |
| Reference paper | Int J Pancreatol 1993;13(1):31-41 - DOI: 10.1007/BF02795197 - PMID: 8454916 |
| Morphology and growth | continuous culture, grown as monolayer, morphology epithelial |
| Culture conditions | MEM (EBSS) + 10% FBS + 2mM L-Glutamine + 1mM Na pyruvate + 1% non-essential amino acids; split confluent cultures 1:2-1:3 using trypsin/EDTA; seed at 2-4 x 104 cells/cm2; 37C, 5% CO2 |
| Properties | CEA and CA19-9 production |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as human with MD, MPI, NP |
| Karyology | modal number 77 |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |
| Comments | incubate with trypsin/EDTA at 37C for at least 10-15 min. After thawing the viability of the cells may be low. |