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Cell line nameA-704
Accession numberICLC HTL95007
Brief descriptionSpecies: human; Tissue: kidney; Tumor: adenocarcinoma
DescriptionSpecies: human male 78 years old; Tissue: kidney; Tumor: adenocarcinoma
DepositorObtained from ECACC.
Reference paperJ Natl Cancer Inst 1973;51:1417-1423 - PMID: 4357758
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial-like
Culture conditionsMEM (EBSS) + 10% FBS + 2mM L-Glutamine + 1mM Na pyruvate + 1% non-essential amino acids; Split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 1-2x105 cells/cm2. 37C, 5% CO2.
Propertieschemotherapeutic agents; cytotoxicity; tumorigenicity
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Species validationValidated by isoenzymes: confirmed as human with MD, MPI
Passage number53
Tumorigenicitynon tumorigenic
Karyologydiploid to hyperdiploid
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Further bibliographyOncogene 1994;9(3):939-942 - PMID: 8108138
Proc Natl Acad Sci USA 1987;84(22):7972-7976 - PMID: 2825172

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