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Cell line nameCOLO 741
Accession numberICLC HTL95008
Brief descriptionSpecies: human; Tissue: colon; Tumor: carcinoma
DescriptionSpecies: human female 69 years; Tissue: colon; Tumor: carcinoma; Derived from: pelvic wall metastasis
DepositorObtained from ECACC
Reference paperUnknown
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial
Culture conditionsRPMI 1640 + 10% FBS + 2mM L-Glutamine; Split confluent cultures 1:2-1:4 using trypsin/EDTA; seed at 3-5 x 104 cells/cm2; 37C, 5% CO2
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Species validationValidated by isoenzymes: confirmed as human with MD, NP
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.

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