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Cell line nameHL-60
Accession numberICLC HTL95010
Brief descriptionSpecies: human, Caucasian; Tissue: peripheral blood; Tumor: leukemia, promyelocytic
DescriptionSpecies: human, Caucasian female 36 years old; Tissue: peripheral blood; Tumor: leukemia, promyelocytic
DepositorObtained from ECACC.
Reference paperNature 1977;270:347-349 - PMID: 271272
Morphology and growthcontinuous culture, grown in suspension, morphology lymphoblast-like
Culture conditionsRPMI 1640 + 10% FBS + 2mM L-Glutamine; split confluent cultures 1:5-1:10; maintain cultures between 5-9x105 cells/ml; 37C, 5% CO2.
Propertiesphagocytosis; antitumour testing; differentiation; pharmacodynamics; cloning; Susceptible to: HIV-1, HTLV-1
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Species validationValidated by isoenzymes: confirmed as human with MD, MPI, NP
Tumorigenicitytumorigenic in nude mice
Karyologypseudodiploid
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Further bibliographyBlood 1979;54:713-733 - PMID: 288488
Proc Natl Acad Sci USA 1978;75:2458-2462 - PMID: 276884
CommentsAfter treatment with DMSO the cells differentiate along the granulocytic line (after 4 days in 1,25% DMSO, 72% of the cells become CD11b positive).

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