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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | HL-60 |
| Accession number | ICLC HTL95010 |
| Brief description | Species: human, Caucasian; Tissue: peripheral blood; Tumor: leukemia, promyelocytic |
| Description | Species: human, Caucasian female 36 years old; Tissue: peripheral blood; Tumor: leukemia, promyelocytic |
| Depositor | Obtained from ECACC. |
| Reference paper | Nature 1977;270:347-349 - DOI: 10.1038/270347a0 - PMID: 271272 |
| Morphology and growth | continuous culture, grown in suspension, morphology lymphoblast-like |
| Culture conditions | RPMI 1640 + 10% FBS + 2mM L-Glutamine; split confluent cultures 1:5-1:10; maintain cultures between 5-9x105 cells/ml; 37C, 5% CO2. |
| Properties | phagocytosis; antitumour testing; differentiation; pharmacodynamics; cloning; Susceptible to: HIV-1, HTLV-1 |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as human with MD, MPI, NP |
| Tumorigenicity | tumorigenic in nude mice |
| Karyology | pseudodiploid |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |
| Further bibliography | Blood 1979;54:713-733 - PMID: 288488
Proc Natl Acad Sci USA 1978;75:2458-2462 - DOI: 10.1073/pnas.75.5.2458 - PMID: 276884 |
| Comments | After treatment with DMSO the cells differentiate along the granulocytic line (after 4 days in 1,25% DMSO, 72% of the cells become CD11b positive). |