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Interlab Cell Line Collection (ICLC)
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Cell line nameDLD-1
Accession numberICLC HTL95011
Brief descriptionSpecies: human; Tissue: colon; Tumor: adenocarcinoma
DescriptionSpecies: human; Tissue: colon; Tumor: adenocarcinoma
DepositorObtained from ECACC.
Reference paperCancer Res 1979;39:1020-1025 - PMID: 427742
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial
Culture conditionsRPMI 1640 + 10% FBS + 2mM L-Glutamine; Split confluent cultures 1:3-1:10 using trypsin/EDTA; seed at 2-3x104 cells/cm2; 37 °C, 5% CO2
DistributionCell line available for distribution. For non-commercial investigative use only
Species validationValidated by isoenzymes: confirmed as human with G6PD, MD, NP
Tumorigenicitytumorigenic in nude mice
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Amelogenin: X,Y; vWA: 18,19; FGA: 22; TH01: 7,9.3; D18S51: 11,17; D21S11: 29,32.2; D8S1179: 15

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