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Cell line nameSH-SY5Y
Accession numberICLC HTL95013
Brief descriptionSpecies: human, Caucasian; Tumor: neuroblastoma
DescriptionSpecies: human, Caucasian female 4 years old; Tumor: neuroblastoma; Derived from: SK-N-SH, originally derived from metastasis to bone marrow
DepositorObtained from ECACC.
Reference paperUnknown
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial-like
Culture conditionsMEM (EBSS) + Hams F12 (1:1) + 10% FBS + 2mM L-Glutamine; Split confluent cultures 1:5-1:10 using trypsin/EDTA; seed at 1x103-1x104 cells/cm2. 37C, 5% CO2.
Propertiesneurotransmitter studies; convert glutamate to GABA; dopamine-beta-hydroxylase activity
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Species validationValidated by isoenzymes: confirmed as human with AST, NP
Passage number14
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Further bibliographyCancer Res 1973;33:2643-2652 - PMID: 4748425
Cancer Res 1978;38:3751-3757 - PMID: 29704
Cancer Res 1990;50:3422-3428 - PMID: 2334938

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