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Cell line nameHeLa 432
Accession numberICLC HTL95016
Brief descriptionSpecies: human, Black; Tissue: cervix; Tumor: carcinoma, epitheloid; Transfected with: ER-pBact; Original line: HeLa
DescriptionSpecies: human, Black female 31 years old; Tissue: cervix; Tumor: carcinoma, epitheloid; Transfected with the exon 4 defective variant of ER-pBact cDNA, human estrogen receptor cDNA, under control of the human beta actin promotor, resistant to G418S.; Original line: HeLa
DepositorDr. U. Pfeffer, Molecular Biology Dept IST at ABC, Genoa, Italy.
Reference paperUnknown
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial-like
Culture conditionsDMEM + 10% FBS + 2mM L-Glutamine; Split confluent cultures 1:2-1:6 using trypsin/EDTA; seed at 1-3x104 cells/cm2; 37C, 5% CO2.
Propertiesvariant estrogen receptor transgene
DistributionCell line available for distribution upon consent of the depositor. For non-commercial investigative use only
Species validationValidated by isoenzymes: confirmed as human with G6PD, NP
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.

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