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Cell line nameMCF7-432
Accession numberICLC HTL95019
Brief descriptionSpecies: human, Caucasian; Tissue: breast; Tumor: adenocarcinoma; Transfected with: ER-pBact cDNA.; Original line: MCF7
DescriptionSpecies: human, Caucasian female 69 years old; Tissue: breast; Tumor: adenocarcinoma; Transfected with the exon 4 defective variant of ER-pBact cDNA, human estrogen receptor cDNA, under control of the human beta actin promotor resistant to G418S.; Original line: MCF7; Derived from: pleural effusion
DepositorDr U. Pfeffer, Molecular Biology Dept, IST at ABC, Genoa Italy.
Reference paperUnknown
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial-like
Culture conditionsDMEM + 10% FBS + 2mM L-Glutamine; Split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 2-4x104 cells/cm2; 37C, 5% CO2.
Propertiesvariant estrogen receptor transgene
DistributionCell line available for distribution upon consent of the depositor. For non-commercial investigative use only
Hazard
Species validationValidated by isoenzymes: confirmed as human with G6PD, NP
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.

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