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Cell line nameHeLa S3
Accession numberICLC HTL95020
Brief descriptionSpecies: human, Black; Tissue: cervix; Tumor: carcinoma, epitheloid
DescriptionSpecies: human, Black female 31 years old; Tissue: cervix; Tumor: carcinoma, epitheloid; Derived from: HeLa
DepositorObtained from ECACC.
Reference paperJ Exp Med 1956;103:273
Morphology and growthcontinuous culture, grown semiadherent, morphology epithelial
Culture conditionsHam's F12 + 10% FBS + 2mM L-Glutamine; Semiadherent cell line, adaptable to growth in suspension (spinner) culture. Maintain culture between 3 and 9x105 cells/ml; split 1:2-1:4. 37C, 5% CO2
Propertiestoxicity screening; virology; bacterial adhesiveness; bacterial invasiveness; enzymology; tumorigenicity; Susceptible to: adenovirus 5, poliovirus 1, vesic. stomatitis (Indiana)
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Species validationValidated by isoenzymes: confirmed as human with AST, G6PD, MD, NP, PepB
Tumorigenicitynon tumorigenic
Karyologymodal number 68
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.

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