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Cell line nameMCF7
Accession numberICLC HTL95021
Brief descriptionSpecies: human, Caucasian; Tissue: breast; Tumor: adenocarcinoma
DescriptionSpecies: human, Caucasian female 69 years old; Tissue: breast; Tumor: adenocarcinoma; Derived from: pleural effusion
DepositorDr U. Pfeffer, Molecular Biology Dept, IST at ABC, Genoa Italy.
Reference paperJ Natl Cancer Inst 1973;51:1409-1416 - PMID: 4357757
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial-like
Culture conditionsDMEM + 10% FBS + 2mM L-Glutamine; Split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 2-4x104 cells/cm2; 37C, 5% CO2.
Virusestype C, type B
Propertiesestrogen receptor positive; specialized product secretion; antitumour testing; cytotoxicity; chemosensibility; chemotherapeutic agents; receptor; hormone sensibility; tumorigenicity
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Species validationValidated by isoenzymes: confirmed as human with G6PD, NP
Tumorigenicitytumorigenic in nude mice
Karyologyhypertriploid to hypotetraploid
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Further bibliographyCancer Res 1990;118:48 - PMID: 2152773
CommentsThe viability may be low after thawing; thaw with medium supplemented with 20% FBS

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