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Cell line nameT84
Accession numberICLC HTL95022
Brief descriptionSpecies: human; Tissue: colon; Tumor: carcinoma
DescriptionSpecies: human 72 years old; Tissue: colon; Tumor: carcinoma; Derived from: metastasis to lung
DepositorObtained from ECACC.
Reference paperUnknown
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial-like
Culture conditionsDMEM + Ham's F12 (1:1) + 10% FBS + 2mM L-Glutamine; Split confluent cultures 1:2-1:3 using trypsin/EDTA; the cells are difficult to disaggregate after tripsinization; seed at 1-3x104 cells/cm2. 37C, 5% CO2.
Propertieshormone studies; tumorigenicity; receptors for many peptide hormones and neurotransmitters; the cells maintain vectorial electrolyte transport.
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Species validationValidated by isoenzymes: confirmed as human with AST, G6PD
Passage number60
Karyologymodal chromosome number 56
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Further bibliographyAm J Physiol 1984;246:204 - PMID: 6141741
Proc Natl Acad Sci USA 1980;77:3464 - PMID: 6932031

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