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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | T84 |
| Accession number | ICLC HTL95022 |
| Brief description | Species: human; Tissue: colon; Tumor: carcinoma |
| Description | Species: human 72 years old; Tissue: colon; Tumor: carcinoma; Derived from: metastasis to lung |
| Depositor | Obtained from ECACC. |
| Reference paper | Unknown |
| Morphology and growth | continuous culture, grown as monolayer, morphology epithelial-like |
| Culture conditions | DMEM + Ham's F12 (1:1) + 10% FBS + 2mM L-Glutamine; Split confluent cultures 1:2-1:3 using trypsin/EDTA; the cells are difficult to disaggregate after tripsinization; seed at 1-3x104 cells/cm2. 37C, 5% CO2. |
| Properties | hormone studies; tumorigenicity; receptors for many peptide hormones and neurotransmitters; the cells maintain vectorial electrolyte transport. |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as human with AST, G6PD |
| Passage number | 60 |
| Karyology | modal chromosome number 56 |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |
| Further bibliography | Am J Physiol 1984;246:204 - DOI: 10.1152/ajpgi.1984.246.2.G204
- PMID: 6141741
Proc Natl Acad Sci USA 1980;77:3464 - DOI: 10.1073/pnas.77.6.3464 - PMID: 6932031 |