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Interlab Cell Line Collection (ICLC)
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Cell line nameDAUDI
Accession numberICLC HTL95024
Brief descriptionSpecies: human, Black; Tumor: lymphoma, Burkitt
DescriptionSpecies: human, Black male 16 years old; Tumor: lymphoma, Burkitt
DepositorObtained from ECACC.
Reference paperCancer Res 1968;28:1300-1310 - PMID: 4174339
Morphology and growthcontinuous culture, grown in suspension, morphology lymphoblast-like
Culture conditionsRPMI 1640 + 10% FBS + 2mM L-Glutamine; Maintain cultures between 2 and 9 x 105 cells/ml; 37C, 5% CO2
Propertiescomplement receptor; Fc receptor; tumor markers; target line; differentiation; antitumour testing; tumorigenicity; virology
DistributionCell line available for distribution. For non-commercial investigative use only
HazardHandle under Biosafety Level 2 containment
Species validationValidated by isoenzymes: confirmed as human with MD, NP
Validated by HLA-DP beta: confirmed as human by PCR
Tumorigenicitytumorigenic in nude mice
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Further bibliographyClin Exp Immunol 1976;25:367-368 - PMID: 963908
Int J Cancer 1977;19:334-337 - DOI: 10.1016/0006-291x(75)90293-4 J Exp Med 1976;143:1528-1533 - DOI: 10.1084/jem.143.6.1528 - PMID: 1083890
Tissue Antigens 1978;11:96-112 - DOI: 10.1111/j.1399-0039.1978.tb01233.x - PMID: 77067

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