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Cell line nameHCT-116
Accession numberICLC HTL95025
Brief descriptionSpecies: human; Tissue: colon; Tumor: carcinoma
DescriptionSpecies: human male; Tissue: colon; Tumor: carcinoma
DepositorObtained from ECACC
Reference paperCancer Res 1981;41:1751-1756 - PMID: 7214343
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial
Culture conditionsMcCoy's 5A + 10% FBS + 2mM L-Glutamine; Split confluent cultures 1:3-1:10 using trypsin/EDTA; seed at 2-4x104 cells/cm2; 37C, 5% CO2
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Species validationValidated by isoenzymes: confirmed as human with MD, NP
Tumorigenicitytumorigenic in nude mice
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Further bibliographyJ Natl Cancer Inst 1982;69:767-771 - PMID: 6956756

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