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Cell line nameSK-N-AS
Accession numberICLC HTL95026
Brief descriptionSpecies: human; Tumor: neuroblastoma
DescriptionSpecies: human female; Tumor: neuroblastoma
DepositorObtained from ECACC.
Reference paperUnknown
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial-like
Culture conditionsDMEM + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; Split confluent cultures 1:2-1:4 using trypsin/EDTA; seed at 1-2x104 cells/cm2. 37C, 5% CO2.
Propertiesvimentin receptor, insulin-like growth factor (IGF-I) receptor, insulin-like growth factor II (IGF-II) synthesis
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Species validationValidated by isoenzymes: confirmed as human with MD, MPI, NP
Tumorigenicitytumorigenic in nude mice
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Further bibliographyCancer Res 1995;55(11):2380-2386 - PMID: 7757990
Cell Growth Differ 1991;2:487 - PMID: 1751405
J Clin Invest 1989;84:829-839 - PMID: 2547840
J Clin Invest 1991;87(2):648-657 - PMID: 1991849

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