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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | SK-N-AS |
| Accession number | ICLC HTL95026 |
| Brief description | Species: human; Tumor: neuroblastoma |
| Description | Species: human female; Tumor: neuroblastoma |
| Depositor | Obtained from ECACC. |
| Reference paper | Unknown |
| Morphology and growth | continuous culture, grown as monolayer, morphology epithelial-like |
| Culture conditions | DMEM + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; Split confluent cultures 1:2-1:4 using trypsin/EDTA; seed at 1-2x104 cells/cm2. 37C, 5% CO2. |
| Properties | vimentin receptor, insulin-like growth factor (IGF-I) receptor, insulin-like growth factor II (IGF-II) synthesis |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as human with MD, MPI, NP |
| Tumorigenicity | tumorigenic in nude mice |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |
| Further bibliography | Cancer Res 1995;55(11):2380-2386 - PMID: 7757990
Cell Growth Differ 1991;2:487 - PMID: 1751405 J Clin Invest 1989;84:829-839 - DOI: 10.1172/JCI114243 - PMID: 2547840 J Clin Invest 1991;87(2):648-657 - DOI: 10.1172/JCI115042 - PMID: 1991849 |