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Cell line nameCOLO 320DMF
Accession numberICLC HTL95027
Brief descriptionSpecies: human; Tissue: colon; Tumor: adenocarcinoma
DescriptionSpecies: human female 55 years old; Tissue: colon; Tumor: adenocarcinoma
DepositorObtained from ECACC
Reference paperCancer Res 1979;39:4914-4924 - PMID: 498117
Morphology and growthcontinuous culture, grown semiadherent, morphology undifferentiated, round cells in suspension and loosely adherent cells in monolayer
Culture conditionsRPMI 1640 + 10% FBS + 2mM L-Glutamine; Split confluent cultures 1:3-1:6: trypsin/EDTA is not necessary as cells are partly in suspension and partly only loosely adherent; seed at 1-3 x 104 cells/cm2; 37C, 5% CO2
Propertiesproduction of hormones: epinephrine, serotonin, norepinephrine, adrenocorticotropic, parathyroid hormone
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Species validationValidated by isoenzymes: confirmed as human with MD, MPI
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Further bibliographyCancer Res 1993;53:5600-5604 - PMID: 8242610

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