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Cell line nameHGC-27
Accession numberICLC HTL95028
Brief descriptionSpecies: human; Tissue: stomach; Tumor: carcinoma, undifferentiated
DescriptionSpecies: human; Tissue: stomach; Tumor: carcinoma, undifferentiated
DepositorObtained from ECACC.
Reference paperActa Med Okayama 1976;30:215-219 - PMID: 136873
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial
Culture conditionsMEM (EBSS) + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 2-4x104 cells/cm2; 37C, 5% CO2.
Propertiesmucin production
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Species validationValidated by isoenzymes: confirmed as human with MD, NP
Freezing mediumMEM + 20% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Amelogenin: X; vWA: 14; FGA: 22; TH01: 9; D18S51: 16; D21S11: 30,33,34; D8S1179: 11
Further bibliographyDig Dis Sci 1997;42:1394-1399 - PMID: 9246035
Scand J Gastroenterol 1999;34:79-84 - PMID: 10048737

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