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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | HGC-27 |
| Accession number | ICLC HTL95028 |
| Brief description | Species: human; Tissue: stomach; Tumor: carcinoma, undifferentiated |
| Description | Species: human; Tissue: stomach; Tumor: carcinoma, undifferentiated |
| Depositor | Obtained from ECACC. |
| Reference paper | Acta Med Okayama 1976;30:215-219 - PMID: 136873 |
| Morphology and growth | continuous culture, grown as monolayer, morphology epithelial |
| Culture conditions | MEM (EBSS) + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 2-4x104 cells/cm2; 37C, 5% CO2. |
| Properties | mucin production |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as human with MD, NP |
| Freezing medium | MEM + 20% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. Amelogenin: X; vWA: 14; FGA: 22; TH01: 9; D18S51: 16; D21S11: 30,33,34; D8S1179: 11 |
| Further bibliography | Dig Dis Sci 1997;42:1394-1399 - DOI: 10.1023/a:1018837920769
- PMID: 9246035
Scand J Gastroenterol 1999;34:79-84 - DOI: 10.1080/00365529950172871 - PMID: 10048737 |