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Cell line nameCaki-2
Accession numberICLC HTL95029
Brief descriptionSpecies: human, Caucasian; Tissue: kidney; Tumor: carcinoma
DescriptionSpecies: human, Caucasian male 69 years old; Tissue: kidney; Tumor: carcinoma
DepositorObtained from ECACC.
Reference paperHuman tumor cells in vitro. Fogh J. ed., Plenum Press, New York, 1975; pp. 115-159
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial-like
Culture conditionsMcCoy's 5A + 10% FBS + 2mM L-Glutamine; Split confluent cultures 1:2-1:4 using trypsin/EDTA; seed at 2-4x104 cells/cm2. 37C, 5% CO2
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Species validationValidated by isoenzymes: confirmed as human with AST, MD, NP, PepB
Passage number52
Tumorigenicitytumorigenic in nude mice
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Further bibliographyJ Natl Cancer Inst 1977;49:5 J Natl Cancer Inst 1977;58:209-214 - PMID: 833871
J Natl Cancer Inst 1977;59:221-226 - PMID: 327080
Natl Cancer Inst Monogr 1978;49:5-19 - PMID: 571047

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