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Cell line nameCOLO 853
Accession numberICLC HTL96001
Brief descriptionSpecies: human; Tissue: lymph node; Tumor: melanoma
DescriptionSpecies: human male 45 years old; Tissue: lymph node; Tumor: melanoma
DepositorObtained from ECACC
Reference paperUnknown
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial
Culture conditionsRPMI 1640 + 10% FBS + 2mM L-Glutamine; Split confluent cultures 1:2-1:4 using trypsin/EDTA; seed at 3-5x104 cells/cm2; 37C, 5% CO2
Propertiesmelanin production
DistributionCell line available for distribution. For non-commercial investigative use only
Species validationValidated by isoenzymes: confirmed as human with AST, MD
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Further bibliographyCancer Genet Cytogenet 1993;69:108-112 - DOI: 10.1016/0165-4608(93)90083-x - PMID: 8402545

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