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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | FTC-238 |
| Accession number | ICLC HTL96003 |
| Brief description | Species: human; Tissue: thyroid; Tumor: carcinoma, follicular |
| Description | Species: human male 42 years old; Tissue: thyroid; Tumor: carcinoma, follicular; Derived from: lung metastasis |
| Depositor | Obtained from ECACC. |
| Reference paper | Unknown |
| Morphology and growth | continuous culture, grown as monolayer, morphology polygonal flat to spindle-like |
| Culture conditions | DMEM + Ham's F12 (1:1) + 5% FBS + 2 mM L-Glutamine; Split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 1-4 x 104 cells/cm2. 37C, 5% CO2. |
| Properties | The cells were used for studies on the function of human thyroid, development of thyroid cancer, expression of 5-deiodinase type I |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as human with MD, NP |
| Passage number | 53 |
| Karyology | complex chromosomal changes; p53 mutation |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |
| Further bibliography | Cancer Res 1990;118:48 - PMID: 2152773
Front Horm Res 1989;18:56 - DOI: 10.1016/0006-291x(75)90293-4 |