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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | HuP-T3 |
| Accession number | ICLC HTL96006 |
| Brief description | Species: human, Japanese; Tissue: pancreas; Tumor: adenocarcinoma |
| Description | Species: human, Japanese male 66 years old; Tissue: pancreas; Tumor: adenocarcinoma; Derived from: ascitic fluid |
| Depositor | Obtained from ECACC. |
| Reference paper | Int J Pancreatol 1993;13(1):31-41 - DOI: 10.1007/BF02795197 - PMID: 8454916 |
| Morphology and growth | continuous culture, grown as monolayer, morphology epithelial |
| Culture conditions | MEM (EBSS) + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids + 1mM Na pyruvate; Split confluent cultures 1:4-1:10 using trypsin/EDTA(10-15 min); seed at 2-4x104 cells/cm2; 37C, 5% CO2 |
| Properties | CEA production |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as human with AST, NP |
| Karyology | modal chromosome number 37 |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |