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Cell line nameHuP-T3
Accession numberICLC HTL96006
Brief descriptionSpecies: human, Japanese; Tissue: pancreas; Tumor: adenocarcinoma
DescriptionSpecies: human, Japanese male 66 years old; Tissue: pancreas; Tumor: adenocarcinoma; Derived from: ascitic fluid
DepositorObtained from ECACC.
Reference paperInt J Pancreatol 1993;13(1):31-41 - PMID: 8454916
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial
Culture conditionsMEM (EBSS) + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids + 1mM Na pyruvate; Split confluent cultures 1:4-1:10 using trypsin/EDTA(10-15 min); seed at 2-4x104 cells/cm2; 37C, 5% CO2
PropertiesCEA production
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Species validationValidated by isoenzymes: confirmed as human with AST, NP
Karyologymodal chromosome number 37
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.

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