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Interlab Cell Line Collection (ICLC)
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Cell line nameMES-SA
Accession numberICLC HTL96008
Brief descriptionSpecies: human, Caucasian; Tissue: uterus; Tumor: sarcoma
DescriptionSpecies: human, Caucasian female 56 years old; Tissue: uterus; Tumor: sarcoma
DepositorObtained from ECACC.
Reference paperCancer Res 1983;43:4943-4950 - PMID: 6883344
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial-like
Culture conditionsMcCoy's 5A + 10% FBS + 2mM L-Glutamine; Split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 2-4x104 cells/cm2; 37C, 5% CO2.
Propertieschemotherapeutic agents; multiple drug-resistant
DistributionCell line available for distribution. For non-commercial investigative use only
Species validationValidated by isoenzymes: confirmed as human with AST, MD, MPI
Tumorigenicitytumorigenic in nude mice
Karyologymodal chromosome number 45
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.

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