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Cell line nameNCI-H292
Accession numberICLC HTL96009
Brief descriptionSpecies: human, Black; Tissue: lung; Tumor: carcinoma, mucoepidermoid
DescriptionSpecies: human, Black female 32 years old; Tissue: lung; Tumor: carcinoma, mucoepidermoid; Derived from: lymph node metastasis
DepositorObtained from ECACC
Reference paperCancer Res 1985;45:2913-2923 - PMID: 2985257
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial
Culture conditionsRPMI 1640 + 10% FBS + 2mM L-Glutamine; Split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 2-6 x 104 cells/cm2; 37C, 5% CO2. After thawing the viability of the cells may be low.
Propertiesvirus replication.; Susceptible to: hepatitis B
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Species validationValidated by isoenzymes: confirmed as human with AST, MD, MPI
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Further bibliographyCancer Res 1985;45:1187-1197 - PMID: 2578876
Science 1983;222:385-389 - PMID: 6194563

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