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Cell line nameBxPC3
Accession numberICLC HTL96011
Brief descriptionSpecies: human; Tissue: pancreas; Tumor: adenocarcinoma
DescriptionSpecies: human female 61 year old; Tissue: pancreas; Tumor: adenocarcinoma
DepositorObtained from ECACC.
Reference paperCancer Invest 1986;4:15 - PMID: 3754176
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial
Culture conditionsRPMI 1640 + 10% FBS + 2mM L-Glutamine; Split confluent cultures 1:3-1:6 using trypsin/EDTA (allow 10 - 15 min. to detach); seed at 2-4 x 104 cells/cm2. 37C, 5% CO2.
Propertiesmucin production
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Species validationValidated by isoenzymes: confirmed as human with MD, NP
Passage number19
Tumorigenicitytumorigenic in nude mice
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Further bibliographyClin Lab Med 1982;2:567-578 - PMID: 6754226

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