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Cell line nameRPMI 7932
Accession numberICLC HTL96012
Brief descriptionSpecies: human; Tissue: skin; Tumor: melanoma
DescriptionSpecies: human; Tissue: skin; Tumor: melanoma
DepositorObtained from ECACC.
Reference paperUnknown
Morphology and growthcontinuous culture, grown as monolayer, morphology fibroblast-like
Culture conditionsRPMI 1640 + 10% FBS + 2mM L-Glutamine; Split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 2-4 x 104 cells/cm2. 37C, 5% CO2.
PropertiesTPA production
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Species validationValidated by isoenzymes: confirmed as human with NP, Pep B
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Further bibliographyCancer Res 1977;37:4660-4668 - PMID: 72600
Cancer Res 1994;54:5917-5924 - PMID: 7954423

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