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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | GI-LI-N |
| Accession number | ICLC HTL96013 |
| Brief description | Species: human, Caucasian; Tumor: neuroblastoma |
| Description | Species: human, Caucasian male 23 months old; Tumor: neuroblastoma |
| Depositor | Dr G.P. Tonini, Population Genetics Dept, IST at ABC, Genoa, Italy |
| Reference paper | J Cancer Res Clin Oncol 1988;114:636-640 - DOI: 10.1007/BF00398190 - PMID: 3204111 |
| Morphology and growth | continuous culture, grown as monolayer, morphology neuroblast-like |
| Culture conditions | RPMI 1640 + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; Split 1:5-1:10 using trypsin/EDTA; seed at 2-4 x 104 cells/cm2. Small and fast growing cells, cell harvest is about 16 x 106/75 cm2 flask. 37C, 5% CO2. |
| Properties | neurotransmitter and catecholamine production, neurobiochemistry, differentiation; tumor necrosis factor assay; interferon induction; N-myc expression undetectable. |
| Distribution | Cell line available for distribution upon consent of the depositor. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as human with MD, NP |
| Tumorigenicity | tumorigenic in nude mice |
| Karyology | chromosome markers: 1p del HSR |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |
| Further bibliography | Pediatr Res 1990;27:1-6 - DOI: 10.1203/00006450-199001000-00001
- PMID: 2296463
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