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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | KB |
| Accession number | ICLC HTL96014 |
| Brief description | Species: human; Tissue: cervix; Tumor: carcinoma |
| Description | Species: human male Adult; Tissue: cervix; Tumor: carcinoma |
| Depositor | Obtained from ECACC. |
| Reference paper | Proc Soc Exp Biol Med 1955;89:362 - DOI: 10.1016/0006-291x(75)90293-4 |
| Morphology and growth | continuous culture, grown as monolayer, morphology epithelial |
| Culture conditions | MEM (EBSS) + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; Split confluent cultures 1:3-1:10 using trypsin/EDTA; seed at 1-4x104 cells/cm2; 37C, 5% CO2. |
| Properties | nutrition; metabolism; chemotherapeutic agents; tumorigenicity; virology; Susceptible to: adenovirus 3, poliovirus 1, vaccinia, herpes simplex, influenza |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | Handle under Biosafety Level 2 containment |
| Species validation | Validated by isoenzymes: confirmed as human with MD, MPI, NP |
| Passage number | 394 |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |
| Further bibliography | EMBO J 1984;3:1151-1157 - PMID: 6329740
Proc Soc Exp Biol Med 1965;120:361 - DOI: 10.3181/00379727-120-30537 - PMID: 4285453 |
| Comments | NB. Originally derived from an epidermoid carcinoma of the mouth of an adult male Caucasian. This cell line was found to be indistinguishable from HeLa by STR PCR DNA profiling |