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Cell line nameSK-N-BE(2)
Accession numberICLC HTL96015
Brief descriptionSpecies: human, Caucasian; Tissue: bone marrow; Tumor: neuroblastoma
DescriptionSpecies: human, Caucasian male 22 months old; Tissue: bone marrow; Tumor: neuroblastoma
DepositorObtained from ECACC
Reference paperScience 1976;191:185-187 - PMID: 942798
Morphology and growthcontinuous culture, grown as monolayer, morphology neuroblast-like and epithelial-like, at confluence forms loosely adherent aggregates
Culture conditionsMEM (EBSS) + Ham's F12 (1:1) + 10% FBS + 2 mM L-Glutamine + 1% non-essential amino acids; Split confluent cultures 1:5-1:10 using trypsin/EDTA; seed at 2-4x104 cells/cm2. 37C, 5% CO2.
Propertiesthe cells are multipotential with regard to neuronal enzyme expression and display a high capacity to convert tyrosine to dopamine
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Species validationValidated by isoenzymes: confirmed as human with G6PD, MD, NP
Passage number29
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Further bibliographyCancer Res 1978;38:3751-3757 - PMID: 29704
J Natl Cancer Inst 1976;57:683-695 - PMID: 62055
Life Sci 1991;50:7-11

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