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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | MEG-01 |
| Accession number | ICLC HTL96017 |
| Brief description | Species: human; Tissue: bone marrow; Tumor: leukemia, megakaryoblastic |
| Description | Species: human male 55 years old; Tissue: bone marrow; Tumor: leukemia, megakaryoblastic; Derived from: a patient with blast crisis of Philadelphia (Ph1) chromosome + chronic myelogenous leukemia (CML) |
| Depositor | Obtained from ECACC. |
| Reference paper | Blood 1985;66:1384-1392 - PMID: 2998511 |
| Morphology and growth | continuous culture, grown in suspension, morphology mixed, round and some adherent cells |
| Culture conditions | RPMI 1640 + 10% FBS + 2mM L-Glutamine; Split confluent cultures 1:5-1:10 using trypsin/EDTA; maintain cultures between 2-4x105 cells/ml; 37C, 5% CO2. |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as human with AST, G6PD, PepB |
| Karyology | modal Nø 56-58; hyperdiploid |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |
| Further bibliography | Exp Cell Res 1991;193:223 - DOI: 10.1016/0014-4827(91)90560-h
- PMID: 1995298
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