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Cell line nameSK-N-BE(2)-C
Accession numberICLC HTL96018
Brief descriptionSpecies: human; Tissue: bone marrow; Tumor: neuroblastoma
DescriptionSpecies: human; Tissue: bone marrow; Tumor: neuroblastoma; Derived from: SK-N-BE(2)
DepositorDr P. Montaldo, G. Gaslini Institute, Genoa, Italy.
Reference paperJ Natl Cancer Inst 1976;57:683-695 - PMID: 62055
Morphology and growthcontinuous culture, grown as monolayer, morphology neuroblast-like
Culture conditionsRPMI 1640 + 10% FBS + 2mM L-Glutamine + 1mM Na pyruvate + 1% non-essential amino acids; Split confluent cultures 1:5-1:10 using trypsin/EDTA; seed at 2-4x104 cells/cm2. 37C, 5% CO2.
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Species validationValidated by isoenzymes: confirmed as human with AST, G6PD
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Further bibliographyCancer Res 1978;38:3751-3757 - PMID: 29704
Life Sci 1991;50:7-11 Science 1976;191:185-187 - PMID: 942798

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