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Cell line nameSK-N-SH
Accession numberICLC HTL96019
Brief descriptionSpecies: human, Caucasian; Tumor: neuroblastoma
DescriptionSpecies: human, Caucasian female 4 years old; Tumor: neuroblastoma; Derived from: bone marrow metastasis
DepositorDr P. Montaldo, G. Gaslini Institute, Genoa, Italy.
Reference paperIn Vitro 1973;7:410
Morphology and growthcontinuous culture, grown as monolayer, morphology neuroblast-like and epithelial-like
Culture conditionsRPMI 1640 + 10% FBS + 2mM L-Glutamine + 1mM Na pyruvate + 1% non-essential amino acids; Split confluent cultures 1:5-1:10 using trypsin/EDTA; seed at 2-4x104 cells/cm2. 37C, 5% CO2.
Propertiesreceptor cell line in cell-mediated cytotoxicity
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Species validationValidated by isoenzymes: confirmed as human with AST, G6PD
Tumorigenicitytumorigenic in nude mice
Karyologymodal number 47
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Further bibliographyCancer Res 1973;33:2643-2652 - PMID: 4748425
Proc Natl Acad Sci USA 1978;75:3965-3969 - PMID: 279013

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