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Cell line nameIMR-32
Accession numberICLC HTL96021
Brief descriptionSpecies: human, Caucasian; Tumor: neuroblastoma
DescriptionSpecies: human, Caucasian male 13 months old; Tumor: neuroblastoma
DepositorDr P. Montaldo, G. Gaslini Institute, Genoa, Italy.
Reference paperCancer Res 1970;30:2110 - PMID: 5459762
Morphology and growthcontinuous culture, grown as monolayer, morphology fibroblast-like and neuroblast-like
Culture conditionsRPMI 1640 + 10% FBS + 2mM L-Glutamine + 1mM Na pyruvate + 1% non-essential amino acids; Split confluent cultures 1:3-1:5 using trypsin/EDTA; seed at 2-4x104 cells/cm2; 37C, 5% CO2.
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Species validationValidated by isoenzymes: confirmed as human with AST, MD
Tumorigenicitytumorigenic in nude mice
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
CommentsThe viability can be low after thawing; thaw with medium supplemented with 20% FBS.

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