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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | LAN-5 |
| Accession number | ICLC HTL96022 |
| Brief description | Species: human; Tumor: neuroblastoma |
| Description | Species: human; Tumor: neuroblastoma |
| Depositor | Dr P. Montaldo, G. Gaslini Institute, Genoa, Italy. |
| Reference paper | J Immunol 1982;128:983 - PMID: 6172518 |
| Morphology and growth | continuous culture, grown as monolayer, morphology neuroblast-like |
| Culture conditions | RPMI 1640 + 10% FBS + 2mM L-Glutamine + 1mM Na pyruvate + 1% non-essential amino acids; Split confluent cultures 1:3-1:4 using trypsin/EDTA; seed at 2-4x104 cells/cm2; after thawing the cells look very suffering, tend to form clusters and need about one week to attach; thaw with medium supplemented with 20% FBS; 37C, 5% CO2. |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as human with AST, Pep B |
| Freezing medium | 90% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |
| Further bibliography | J Neurosci Res 1982;8:375-391 - DOI: 10.1002/jnr.490080226
- PMID: 6296415
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| Comments | The cells grow very slowly |